Abcam, ab281598, Human WWTR1 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
WWTR1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-WWTR1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TAZ also known as WWTR1 (WW Domain-Containing Transcription Regulator 1) acts as a transcriptional co-activator in cells. TAZ has a molecular mass of approximately 45 kDa. This protein appears in various tissues including the lung kidney brain and heart. TAZ interacts with other transcription factors to influence gene expression playing a role in cellular signaling and development.
Biological function summary
TAZ works as a part of the Hippo signaling pathway regulating cell growth proliferation and apoptosis. It changes cellular responses through interactions with other proteins such as TEAD transcription factors. TAZ can act within the cytoplasm or the nucleus changing its location in the cell based on physiological signals. It operates independently as well as in concert with other molecules assisting in maintaining tissue homeostasis and regeneration.
Pathways
TAZ functions as an essential element of the Hippo pathway which controls organ size and suppresses cancer. It plays significant roles in the PI3K-AKT signaling pathway coordinating with YAP (Yes-associated protein) a well-known partner in these pathways. TAZ and YAP together influence transcriptional outcomes for numerous genes adjusting to the needs of various cellular contexts which affect cell behavior significantly.
TAZ associates with cancer and fibrosis. In many cancers TAZ exhibits high activity often linked with poor prognosis due to its role in promoting cell proliferation and survival. TAZ interacts with beta-catenin in cancer contexts influencing signaling that drives cancer progression. In fibrosis aberrant TAZ signaling leads to excessive tissue scarring working alongside CTGF (Connective Tissue Growth Factor) to support this pathological development.