Abcam, ab28226, Anti-Calpain 10 antibody

Size: 100µg
Rabbit Polyclonal Calpain 10 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human CAPN10.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human CAPN10. The exact immunogen used to generate this antibody is proprietary information.Q9HC96,
Specificity:The antibody binds to Calpain-10, but does not cross react with the other calpain family members (Calpain-1, Calpain-2, Calpain-3, etc.). The antibody binds to the aminoterminal end of domain-III and recognizes latent and amino-processed Calpain-10.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-Preservative: 0.05% Sodium azideConstituents: 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Calpain 10 also known as CAPN10 is an intracellular cysteine protease enzyme belonging to the calpain family. It has a molecular mass of approximately 75 kDa and shows expression in various tissues including the pancreas skeletal muscle and liver. Calpain 10 plays a mechanical role in modulating cellular processes by cleaving target substrates involved in cellular functions such as signal transduction differentiation and apoptosis. This enzyme requires calcium ions for its activation.
Biological function summary
Calpain 10 contributes to the regulation of intracellular signaling pathways and cellular homeostasis. It plays a role in cytoskeletal remodeling extricating proteins from multiprotein complexes and influencing glucose metabolism. Compared to other calpains Calpain 10 differs structurally as it lacks the typical EF-hand calcium-binding domains. This characteristic suggests its unique mechanism in modulating physiological processes within cells.
Pathways
Calpain 10 interacts with stress-related signaling and metabolic pathways. Particularly it functions within the insulin signaling pathway essential for glucose uptake and metabolism. Calpain 10’s activity affects the insulin receptor substrate (IRS) and is related to the phosphatidylinositol 3-kinase (PI3K) pathway which is important for cellular metabolism and survival. These pathways highlight the relationship between Calpain 10 and metabolic control.
Variations and dysfunctions in Calpain 10 connect to type 2 diabetes and plays a role in risk for the disorder. Genetic studies indicate that mutations and polymorphisms in the CAPN10 gene correlate with obesity and impaired insulin signaling contributing to diabetes susceptibility. Calpain 10 shares a connection with other proteins involved in glucose and lipid metabolism including IRS proteins and glucose transporters which relate to the pathophysiology of these conditions.