Abcam, ab282462, Anti-GRHL2 antibody [EPR24386-76] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal GRHL2 antibody. Carrier free. Suitable for ICC/IF, IP, Flow Cyt (Intra), WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24386-76,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-P, IP, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab282462 is the carrier-free version of
ab271023
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GRHL2 also known as Grainyhead-like protein 2 homolog serves an important role in transcription regulation. This protein has a mass of approximately 68 kDa. It is expressed in various tissues including the liver kidneys and skin. GRHL2 functions mechanically by binding DNA sequences and influencing the expression of genes involved in epithelial development and maintenance.
Biological function summary
GRHL2 plays a critical role in epithelial cell differentiation and proliferation. It is not known to form part of a larger protein complex but rather acts individually to regulate genes associated with epidermal barrier formation and wound healing. GRHL2 affects keratinocyte behavior and has been shown to modulate cellular adhesion molecules like E-cadherin highlighting its direct impact on cellular architecture.
Pathways
GRHL2 influences key regulatory networks such as the Wnt signaling and epithelial-mesenchymal transition (EMT) pathways. Within these systems GRHL2 interacts with proteins like beta-catenin to modulate gene expression important for maintaining epithelial cell identity. These pathways highlight its label as a gatekeeper against unwanted cellular transition states.
Mutations or dysregulation of GRHL2 have been linked to conditions like breast cancer and hereditary hearing loss. In breast cancer aberrant GRHL2 expression affects oncogenes like MYC facilitating tumor growth and metastasis. In hereditary hearing loss GRHL2's impact on E-cadherin and other junctional proteins leads to inner ear developmental defects shedding light on its role in cellular integrity beyond the skin.