Abcam, ab283720, Anti-NFAT1 antibody [EPR24658-43] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NFAT1 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24658-43,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IP, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab283720 is the carrier-free version of
ab283691
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NFAT1 also known as NFATc2 is a transcription factor involved in gene regulation with a molecular weight of approximately 100 kDa. It belongs to the NFAT (nuclear factor of activated T-cells) family and is expressed in a variety of tissues including the immune system and the nervous system. NFAT1 plays an essential role in activating specific gene expressions by translocating into the nucleus upon cell stimulation. Researchers frequently use NFAT molecular weight to confirm its presence in protein analyses such as NFAT western blot techniques.
Biological function summary
NFAT1 influences immune response and development. It acts as part of a large complex with other transcription factors to bind DNA and regulate transcription of cytokines and other critical immune molecules. In activated T-cells NFAT1 controls the expression of genes important for immune function including interleukins. In neuronal tissues it contributes to neural growth and survival highlighting its multifunctional nature in different cell types.
Pathways
NFAT1 integrates into the calcineurin signaling pathway and plays a significant role in T-cell activation. It acts downstream of the calcium signaling cascade which leads to dephosphorylation and activation by the phosphatase calcineurin. NFAT1 works closely with proteins like AP-1 and GATA to facilitate the transcription of immune-related genes. These interactions highlight NFAT1's importance in immune signaling pathways which modulate immune surveillance and homeostasis.
NFAT1 associates with autoimmune diseases and certain cancers. In autoimmune conditions such as rheumatoid arthritis aberrant NFAT1 function leads to excessive cytokine production exacerbating inflammation. The protein also links to cancer development particularly in lymphoid malignancies where dysregulated NFAT1 activity contributes to uncontrolled cell proliferation. It interacts with oncogenic kinases and other dysregulated proteins in these disorders influencing both their progression and possible therapeutic targeting.