Abcam, ab283954, Anti-SARS-COV-2 nsp8 antibody [EPR24854-42]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal R1AB antibody. Suitable for WB, I-ELISA, IHC-P and reacts with SARS-CoV-2 samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24854-42,
Isotype:IgG,
Carrier free:No,
Reacts with:SARS-CoV-2,
Applications:I-ELISA, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SARS-CoV-2 nsp8 also known as non-structural protein 8 forms part of the viral replicase complex of the severe acute respiratory syndrome coronavirus 2. Nsp8 has a mass of approximately 22 kDa and plays an important role in the RNA synthesis process of the virus. It is expressed inside host cells once the virus enters the host organism. The protein functions as a part of the RNA polymerase complex working alongside other non-structural proteins to enable the virus to replicate its genetic material effectively.
Biological function summary
Nsp8 cooperates with non-structural protein 12 (nsp12) to form the RNA-dependent RNA polymerase (RdRp) complex. This complex is essential for the replication and transcription of the viral genome. Nsp8 nsp12 and nsp7 come together as a multi-protein complex enhancing the activity of the RdRp. Nsp8 has been shown to stabilize the structure and function of the replication machinery providing the necessary support for the synthesis of the viral RNA strand.
Pathways
Nsp8 plays an important role in the viral replication and transcription pathways. The polymerase activity driven by the complex of nsp8 nsp12 and nsp7 operates through the replication-transcription complex important for efficient viral propagation within the host. This process not only involves the formation of viral RNA genomes but also subgenomic RNAs which are required for viral protein translation. The interaction between these non-structural proteins ensures that the virus maintains a high level of infectious presence in its host.
Nsp8 holds a strong connection to COVID-19 the disease caused by SARS-CoV-2 infection. The interplay between nsp8 and other non-structural proteins such as nsp12 contributes directly to the ability of the virus to replicate and cause widespread infection. Inhibition of nsp8 function could provide a therapeutic target for treating COVID-19 as disruption of the RdRp complex could hinder the viral replication process. Given the pivotal role of this protein in the virus’s lifecycle targeting nsp8 could offer promising avenues for antiviral drug development.