Abcam, ab283968, Human Nucleoside Diphosphate Kinase D ELISA Kit

Size: 1 x 96Tests
Human Nucleoside Diphosphate Kinase D ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Nucleoside Diphosphate Kinase D in Human in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum, Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.34 ng/mL,
Range:0.75 - 24 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
The Human Nucleoside Diphosphate Kinase D ELISA Kit (ab283968) is designed for detection of Nucleoside Diphosphate Kinase D (NDPKD) in human plasma, serum, cell culture, and cell lysate samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human NDPKD in approximately 5 hours. A polyclonal antibody specific for human NDPKD has been pre-coated onto a 96-well microplate with removable strips. NDPKD in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human NDPKD, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NME4 also known as nucleoside diphosphate kinase D is a mitochondrial protein with a mass of approximately 17 kDa. It is found in the inner mitochondrial membrane and is part of the NDP (nucleoside diphosphate) kinase family. This protein plays a role in transferring gamma-phosphates from nucleoside triphosphates to nucleoside diphosphates facilitating essential cellular energy transactions. NME4's catalytic activity helps maintain the balance and availability of nucleotides within the mitochondria.
Biological function summary
Nucleoside diphosphate kinase activity of NME4 contributes to energy homeostasis in cells. It assists in the generation of nucleoside triphosphates necessary for mitochondrial function and integrity. NME4 operates as part of multimeric complexes within the mitochondria ensuring efficient energy metabolism. Its role extends to contributing to cellular signaling pathways by providing the necessary energy substrates for a variety of cellular responses and activities.
Pathways
Researchers have found that NME4 is involved in the regulation of the mitochondrial apoptotic pathway. It interacts with other proteins involved in apoptosis such as Bcl-2 family members by influencing the mitochondrial outer membrane permeability. Additionally NME4 participates in pathways related to oxidative phosphorylation working alongside other proteins like ATP synthase to optimize energy production and utilization. These interactions underpin its versatility in sustaining cellular energy requirements and influencing cell survival.
Dysfunction in NME4 activity has been linked to mitochondrial diseases and cancer. In particular alterations in NME4 have implications in mitochondrial myopathy where energy production becomes compromised. Similarly cancer studies have shown changes in NME4 expression affect tumor growth and survival often in conjunction with proteins involved in metabolic regulation like p53. Understanding NME4's interactions and modifications can provide insights into its role in these diseases and potential therapeutic targets.