Abcam, ab284005, Human HDHD2 ELISA Kit

Size: 1 x 96Tests
Human HDHD2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human HDHD2 in Human in Biofluids, Cell/Tissue Extracts samples.
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Cell Lysate,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 21 pg/mL,
Range:0.031 - 2 ng/mL,
Assay time:4h,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
The Human HDHD2 ELISA Kit (ab284005) is designed for detection of HDHD2 in human CSF and cell lysate samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human HDHD2 in approximately 4 hours. A polyclonal antibody specific for human HDHD2 has been pre-coated onto a 96-well microplate with removable strips. HDHD2 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for human HDHD2, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HDHD2 also known as haloacid dehalogenase-like hydrolase domain-containing protein 2 is an enzyme with a molecular weight of approximately 22 kDa. It primarily functions as a phosphatase which removes phosphate groups from specific substrates. HDHD2 is expressed in several tissues including the liver and brain. Its activity is integral to regulating phosphate metabolism and maintaining cellular homeostasis.
Biological function summary
HDHD2 hydrolyzes phosphoserines and plays an essential role in dephosphorylation processes. It is a component of protein complexes that control phosphorylation states within cells directly affecting protein functions. Through these actions HDHD2 influences cellular signaling and metabolism contributing to physiological processes such as enzyme regulation and substrate modification.
Pathways
HDHD2 participates in signaling pathways related to phosphate metabolism and energy balance. It is related to the energy production pathway and the regulation of cellular signaling cascades. This protein interacts with other enzymes like PPARG and PTPMT1 coordinating the maintenance of phosphate balance across cellular compartments.
Abnormalities in HDHD2 function can be linked to metabolic disorders including diabetes and obesity. Its disturbance affects phosphate homeostasis and potentially contributes to insulin resistance. HDHD2 interacts with proteins such as insulin receptors and SREBP-1c implicating it in metabolic dysregulation associated with these conditions.