Abcam, ab284018, Human PR3 Autoantibody ELISA Kit

Size: 1 x 96Tests
Human PR3 Autoantibody ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human PR3 Autoantibody in Human in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.44 AU/mL,
Range:1.875 - 30 AU/mL,
Assay time:4h,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
The Human Proteinase 3 Autoantibody ELISA (ab284018) Kit is designed for the quantitative determination of autoimmune response (IgG) to a target antigen (PR3). The kit detects autoantibodies in plasma and serum samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures autoantibodies (anti-PR3 IgG) in approximately 4 hours. A PR3 antigen has been pre-coated onto a 96-well microplate with removable strips. An autoantibody specific for PR3 in standards and samples is sandwiched by the immobilized antigen and a biotinylated polyclonal antibody specific for human IgG, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Proteinase 3 (PR3) also known as PRTN3 is a serine protease with a mass of approximately 29 kDa. It belongs to the human neutrophil elastase (HNE) family and is mainly expressed in neutrophils. Within these immune cells PR3 localizes to azurophilic granules where it participates in early immune responses. The proteolytic activity of PR3 involves cleaving peptide bonds in target proteins contributing to the processing and degradation of various proteins important for immune function.
Biological function summary
Proteinase 3 serves multiple roles including modulating inflammation and immune responses. PR3 functions as part of a complex with azurophilic granules aiding in microbial defense mechanisms. In addition the protease regulates cytokine activation and deactivation which in turn influences cell signaling related to inflammation. Its enzymatic action ensures that PR3 can manage inflammatory processes by breaking down extracellular matrix components thereby allowing white blood cells to access infection sites.
Pathways
Proteinase 3 plays an important role within the serine protease and inflammatory pathways. It interacts with other proteins such as neutrophil elastase with which it shares a pathway to modulate immune responses. PR3 also partners with cathepsin G contributing to processes such as pathogen clearance and tissue remodeling providing a coordinated response during infection and injury. These interactions show PR3's involvement in regulating the balance between inflammation and tissue repair.
PR3 is notably associated with Wegener's granulomatosis also known as granulomatosis with polyangiitis. This autoimmune condition results in systemic vasculitis where anti-proteinase 3 antibodies notably characterize the disease. Additionally PR3 plays a part in chronic obstructive pulmonary disease (COPD) where its proteolytic activity contributes to tissue damage in the lungs. In both conditions PR3 interplays with related proteins like myeloperoxidase further demonstrating its importance in processes leading to these disorders.