Abcam, ab284050, Anti-SARS-CoV-2 nsp2 antibody [EPR24853-4] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal R1AB antibody. Carrier free. Suitable for ICC/IF, IHC-P, Flow Cyt (Intra), ELISA, WB and reacts with Transfected cell line - SARS-CoV-2, SARS-CoV-2 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24853-4,
Isotype:IgG,
Carrier free:Yes,
Reacts with:SARS-CoV-2,
Applications:WB, IHC-P, Flow Cyt (Intra), ICC/IF, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab284050 is the carrier free version of
ab284036
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SARS-CoV-2 nsp2 also known as non-structural protein 2 is a protein encoded by the SARS-CoV-2 virus. It has a predicted mass of around 67 kDa. Nsp2 is expressed during the early stages of the virus replication cycle inside the host cells particularly in the cytoplasm. This protein interacts with host cell components but its functional mechanisms remain partially understood. Studies suggest it might play a role in modifying host cell environment to facilitate viral replication or assembly.
Biological function summary
Within the framework of viral infection processes nsp2 does not form part of a well-defined multimeric complex. However evidence indicates that it can impact host cell signaling pathways. Specifically nsp2 may exert influence on cellular signaling through its interactions with host proteins potentially altering important cellular processes like immune responses. These interactions suggest a possible adaptation strategy that the virus utilizes to maintain persistence and replication advantage within the host organism.
Pathways
The role of nsp2 is implicated in both viral replication and host cell response pathways. For instance nsp2 potentially affects pathways such as the NF-kB signaling pathway known for regulating immune responses and inflammation. It also appears related to the MAPK signaling pathway which controls various cellular activities including growth and differentiation. Within these pathways nsp2 may interact with host proteins like kinases although detailed interaction networks require further investigation.
SARS-CoV-2 nsp2 is most directly connected with COVID-19 the disease caused by the virus. Its possible role in modifying host cell pathways suggests involvement in disease severity possibly linked to immune dysregulation observed in severe cases of the illness. Moreover while nsp2's direct interactions with other viral proteins such as nsp12 the RNA-dependent RNA polymerase are less documented their combined effects significantly impact the virus's ability to replicate and evade host defenses.