Abcam, ab284633, Anti-SARS-CoV-2 nsp6 antibody [EPR24845-90]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal R1AB antibody. Suitable for ICC/IF, Flow Cyt (Intra), WB, I-ELISA and reacts with Transfected cell line - SARS-CoV-2, SARS-CoV-2 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24845-90,
Isotype:IgG,
Carrier free:No,
Reacts with:SARS-CoV-2,
Applications:Flow Cyt (Intra), WB, ICC/IF, I-ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SARS-CoV-2 nsp6 also known as non-structural protein 6 plays an important role in the lifecycle of the virus. This protein is part of the replicase polyprotein and has an estimated molecular weight of about 33 kDa. It is expressed mainly within infected host cells where it localizes to the endoplasmic reticulum. nsp6 helps in modifying host cell membranes to form vesicles which are necessary for replication and transcription of the viral RNA.
Biological function summary
Non-structural protein 6 is involved in the host cell's autophagy process. It interacts with other non-structural proteins like nsp3 and nsp4 to form a complex that transforms host cell membranes. These membrane structures can shield viral components from the host’s immune system. Autophagy inhibition by nsp6 can also help in providing the virus with a platform for its replication processes.
Pathways
SARS-CoV-2 nsp6 participates in the early vesicle formation process critical to the viral replication complex. It works within the autophagy and endoplasmic reticulum stress pathways. In the autophagy pathway nsp6 connects with proteins such as LC3 and ATG5 which control autophagosome formation. This connection allows the virus to evade cellular mechanisms meant to degrade it enhancing viral persistence and replication in host cells.
SARS-CoV-2 nsp6 relates closely to COVID-19. This protein’s interaction with the autophagy process may contribute to the inflammatory response observed during infection. Moreover its functions link to proteins like nsp3 increasing viral replication which can worsen disease progression. This might lead to severe respiratory conditions and has implications in the development of therapies targeting those protein interactions.