Abcam, ab284715, Anti-E1B-AP5 antibody [EP2633Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Monoclonal E1B-AP5 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP2633Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab284715 is the carrier-free version of
ab68480
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The E1B-AP5 protein also known as hnRNPUL1 serves mechanical functions by binding to RNA and DNA and it acts as a regulator in transcription and mRNA processing. This protein weighs approximately 88 kDa. Researchers have identified its presence in the nucleus of different cell types reflecting its central role in nucleic acid metabolism. The protein can also intervene in alternative splicing events and influence mRNA stability making it a significant player within the cell's genetic expression regulation matrix.
Biological function summary
E1B-AP5 integrates into cellular processes as a part of the heterogeneous nuclear ribonucleoproteins complex which facilitates various RNA processing mechanisms. It plays an active role in chromatin structure modulation and RNA transport fostering proper gene expression and maintaining cellular homeostasis. Besides supporting the transcription machinery E1B-AP5 engages in RNA export and surveillance where its presence determines the fate of newly synthesized RNAs.
Pathways
Studies have shown that E1B-AP5 is involved in pathways such as the mRNA surveillance and splicing pathways. It collaborates with proteins like HNRNPA1 and HNRNPC in orchestrating the correct assembly and exportation of RNA transcripts. By participating in these critical pathways E1B-AP5 ensures the fidelity of gene expression and supports the cellular stress response mechanisms contributing to proper cellular function and adaptability.
Abnormalities in E1B-AP5 expression correlate with oncogenic transformations and neurodegenerative conditions. In cancer for example dysregulation of E1B-AP5 affects RNA processing and can lead to tumor development with its interaction noted alongside the protein p53 in the context of cellular proliferation control. Understanding E1B-AP5’s role in these diseases might provide insights into therapeutic targets particularly where RNA processing and tumor suppressor pathways are implicated.