Abcam, ab285231, Mouse Creatine Kinase MB ELISA Kit

Size: 96Test
Mouse Creatine Kinase MB ELISA Kit is a sandwich ELISA designed to quantify Mouse Creatine Kinase MB with a sensitivity of 46.825 pg/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 78.125 - 5000 pg/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Homogenate, Serum, Other biological fluids,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 46.825 pg/mL,
Range:78.125 - 5000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse Creatine Kinase MB ELISA Kit ab285231 is a sandwich ELISA to measure Mouse Creatine Kinase MB in serum, plasma, tissue homogenate, other biological fluids with a sensitivity of 46.825 pg/ml.
How the assay works
The density of color is proportional to the amount of CK-MB captured from the samples.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse Creatine Kinase MB ELISA Kit ab285231 protocol summary
1. Wash plate with wash solution
2. Add standards and samples into appropriate wells. Incubate then discard
3. Add Biotin detection antibody and incubate,
4. Wash
Add SABC solution and incubate
5. Wash
6. Add TMB solution and incubate
7. Add Stop solution and read at 450 nm

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C