Abcam, ab285236, Mouse Osteocalcin ELISA Kit

Size: 96Test
Mouse Osteocalcin ELISA Kit is a sandwich ELISA designed to quantify Mouse Osteocalcin with a sensitivity of 0.23 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 0.39 - 25 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum, Other biological fluids,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.23 ng/mL,
Range:0.39 - 25 ng/mL,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse Osteocalcin ELISA Kit ab285236 is a sandwich ELISA to measure Mouse Osteocalcin in serum, plasma, other biological fluids with a sensitivity of 0.23 ng/ml.
How the assay works
Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for mouse Osteocalcin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of Osteocalcin bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of mouse Osteocalcin. The concentration of mouse Osteocalcin in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse Osteocalcin ELISA Kit ab285236 protocol summary
1. Add standards and samples to appropriate wells.Incubate
2. Add Biotinylated Detection Antibody. Incubate
3. Wash
4. Add HRP Conjugate. Incubate
5. Wash
6. Add Substrate Reagent. Incubate
7. Add stop solution and read at 450 nm
This product is manufactured by BioVision, an Abcam company and was previously called E4763 Osteocalcin (Mouse) ELISA Kit. E4763-100 is the same size as the 96 test size of ab285236.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Osteocalcin also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP) is a small non-collagenous protein with a molecular weight of approximately 5.8 kDa. This protein is mainly expressed in osteoblasts which are bone-forming cells. It is an important component of the bone matrix and is involved in bone mineralization. Osteocalcin binds to calcium and incorporates into the hydroxyapatite crystals in the bone helping stabilize the bone structure. Researchers often study this protein as a marker for bone formation and turnover.
Biological function summary
Osteocalcin plays a significant role in regulating glucose metabolism and fat deposition. It acts as a hormone from the bone and influences energy metabolism and insulin sensitivity. Osteocalcin undergoes carboxylation a post-translational modification necessary for its function in bone. Beyond its structural role demineralized osteocalcin has been found to impact pancreatic β-cell function adipose tissue and male fertility although the pathways in these processes are not entirely understood yet.
Pathways
Osteocalcin is associated with the energy metabolism and bone remodeling pathways. It interacts with the insulin signaling pathway influencing insulin secretion by pancreatic β-cells and boosting insulin sensitivity. This interaction involves proteins like insulin receptor substrate 1 (IRS1) and glucose transporter type 4 (GLUT4) which play roles in glucose uptake. Osteocalcin also connects to bone remodeling pathways by influencing osteoclast differentiation and activity through osteoprotegrin and receptor activator of nuclear factor kappa-B ligand (RANKL).
Dysregulation of osteocalcin levels associates with metabolic syndrome and osteoporosis. It is linked with insulin resistance affecting glucose homeostasis and potentially contributing to diabetes development. Osteocalcin's involvement in bone density maintenance also makes it relevant in osteoporosis where bone formation balance is disrupted. The receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegrin which osteocalcin interacts with are other proteins connected to the pathogenesis of these conditions. Understanding osteocalcin's function helps in exploring therapeutic targets for these diseases.