Abcam, ab285269, Human OxLDL ELISA Kit

Size: 96Test
Human OxLDL ELISA Kit is a sandwich ELISA designed to quantify Human OxLDL with a sensitivity of 1.875 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 3.125 - 200 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:< 1.875 ng/mL,
Range:3.125 - 200 ng/mL,
Assay Platform:Microplate

Product details:
Human OxLDL ELISA Kit ab285269 is a sandwich ELISA to measure Human OxLDL in serum, plasma, cell culture supernatant, tissue homogenate, other biological fluids with a sensitivity of 1.875 ng/ml.
How the assay works
The density of color is proportional to the amount of OxLDL captured from the samples.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Human OxLDL ELISA Kit ab285269 protocol summary
1. Wash plate with wash solution
2. Add standards and samples into appropriate wells. Incubate then discard
3. Add Biotin detection antibody and incubate,
4. Wash
Add SABC solution and incubate
5. Wash
6. Add TMB solution and incubate
7. Add Stop solution and read at 450 nm

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C