Abcam, ab285309, Mouse Superoxide Dismutase 1 ELISA Kit

Size: 96Test
Mouse Superoxide Dismutase 1 ELISA Kit is a sandwich ELISA designed to quantify Mouse Superoxide Dismutase 1 with a sensitivity of 0.469 ng/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 0.781 - 50 ng/mL
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Homogenate, Serum, Other biological fluids,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:< 0.469 ng/mL,
Range:0.781 - 50 ng/mL,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Mouse Superoxide Dismutase 1 ELISA Kit ab285309 is a sandwich ELISA to measure Mouse Superoxide Dismutase 1 in serum, plasma, tissue homogenate, other biological fluids with a sensitivity of 0.469 ng/mL
How the assay works:
The density of color is proportional to the amount of SOD1 captured from the samples.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Mouse Superoxide Dismutase 1 ELISA Kit ab285309 protocol summary
1.Wash plate and add standards or samples to appropriate wells. Incubate
2. Add Biotin-detection antibody work solution to the wells. Incubate
3. Wash the plate
4. Add SABC working solution into each well. Incubate
5. Wash the plate
6. Add TMB substrate to the wells. Incubate
6. Add stop solutionand read at 450 nm

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Superoxide Dismutase 1 (SOD1) also known as Cu/Zn SOD or simply dismutase is an enzyme important for the detoxification of superoxide radicals. SOD1 catalyzes the conversion of two superoxide molecules into oxygen and hydrogen peroxide maintaining cellular redox balance. This enzyme typically exhibits a mass of approximately 32 kDa. SOD1 is expressed in the cytoplasm of cells throughout the body including tissues like liver kidney and brain.
Biological function summary
The enzyme functions as a homodimer with each subunit containing a copper and zinc ion. These metal ions are essential for the catalytic activity of SOD1 as the copper ion participates in electron transfer while the zinc ion provides structural stability. The enzyme protects cells from oxidative stress by neutralizing excess reactive oxygen species ensuring cellular health and functioning.
Pathways
SOD1 plays an important role in the cellular antioxidant defense system and is a part of the reactive oxygen species (ROS) metabolic pathway. It works in conjunction with catalase and glutathione peroxidase to limit oxidative damage within cells. The close interaction between these enzymes highlights the interdependence within the antioxidant defense network emphasizing their role in maintaining cellular homeostasis.
Mutations in the SOD1 gene are linked to familial Amyotrophic Lateral Sclerosis (ALS) a neurodegenerative condition. In ALS aberrant SOD1 proteins may lead to increased oxidative stress and motor neuron damage. Research also connects SOD1 to the pathogenesis of Alzheimer's disease where oxidative stress is a contributing factor. These associations underline SOD1's significance in neurodegenerative diseases highlighting potential therapeutic targets for interventions focused on oxidative stress management.