Abcam, ab2868, Anti-Ryanodine Receptor antibody [34C]

Size: 100µL
Anti-Ryanodine Receptor antibody [34C] (ab2868) is a mouse monoclonal antibody detecting Ryanodine Receptor in Western Blot, IP, IHC-P, IHC-Fr, ICC/IF . Suitable for Cow, Dog, Human, Mouse, , Pig, Rabbit, Rat, Sheep, Xenopus laevis . - Over 80 publications - Trusted since 2003
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:34C,
Isotype:IgG1,
Carrier free:No,
Reacts with:Rat, Human, Primates, Xenopus laevis, Cow, Sheep, Dog, Mouse, Rabbit, Pig,
Applications:IHC-Fr, FuncS (Neut/Block), ICC/IF, IHC-P, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Detects Ryanodine Receptor (RyR)-1 and RyR-2 isoforms.In chickens, this antibody detects the alpha, beta and cardiac isoforms. This antibody also detects RyR-3 in mouse cells. In frog, this antibody detects the alpha and beta isoforms. In fish, this antibody detects the alpha isoform.By Western blot, this antibody detects a 565 kDa protein representing RyR from rat skeletal muscle extracts. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in chicken brain results in intense staining of cerebellar Purkinje neurons.

Product details:
What is this antibody validated in?
Anti-Ryanodine Receptor antibody [34C] (ab2868) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Cow, Dog, Human, Mouse, , Pig, Rabbit, Rat, Sheep, Xenopus laevis samples.
What is the molecular weight of Ryanodine Receptor?
Anti-Ryanodine Receptor [34C] (ab2868) specifically detects a band for Ryanodine Receptor (UniProt: P21817) at a molecular weight of 565kDa.
Trusted by the scientific community
Anti-Ryanodine Receptor [34C] (ab2868) was first used in a scientific publication in 2003 and has been cited over 80 times in peer-reviewed journals.
Reviewed by scientists
Anti-Ryanodine Receptor [34C] (ab2868) has over 10 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ryanodine receptor also known as RyR is a significant calcium ion channel that facilitates the release of calcium from the sarcoplasmic reticulum into the cytosol. It is especially important in cardiac and skeletal muscle cells influencing muscle contraction. The receptor exists in three isoforms: RyR1 RyR2 and RyR3 with RyR1 dominant in skeletal muscle RyR2 in cardiac muscle and RyR3 more ubiquitously in the body. RyR is a large protein with a mass of approximately 2 megadaltons comprising multiple subunits and forming part of a transmembrane complex.
Biological function summary
These receptors regulate calcium ions important for triggering muscle contractions and other calcium-dependent processes. RyR operates as part of a complex with other proteins such as FKBP12 (calstabin) and calmodulin which modulate its function. The receptor plays an important role in excitation-contraction coupling in muscles ensuring proper physiological responses to stimuli. Calcium release through these channels not only manages contraction but also assists in signal transduction within various cellular contexts.
Pathways
The ryanodine receptor integrates significantly into the excitation-contraction coupling and the calcium signaling pathways. In excitation-contraction coupling the receptor responds to electrical stimuli by releasing calcium that initiates contraction. In the calcium signaling pathway it participates by releasing and regulating intracellular calcium level interacting with proteins such as L-type calcium channels and calsequestrin to maintain cell homeostasis. These interactions define the RyR's role in cellular respiration muscle contraction and other physiological processes.
Abnormal function or mutations in RyR often associate with cardiac and skeletal muscle diseases such as malignant hyperthermia and catecholaminergic polymorphic ventricular tachycardia. Dysregulation or mutation-induced alterations affect calcium release and contribute to pathogenesis. Connections between RyR dysfunction and other proteins such as junctin or triadin in muscle cells frequently disrupt normal muscle function illustrating RyR's significant role in these disorders. The receptor's involvement in calcium dysregulation suggests potential avenues for therapeutic interventions.