Abcam, ab286914, Anti-SARS-CoV-2 nsp10 antibody

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal R1AB antibody. Suitable for I-ELISA, WB and reacts with Recombinant fragment - SARS-CoV-2 samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24836-8-8,
Isotype:IgG,
Carrier free:No,
Reacts with:SARS-CoV-2,
Applications:WB, I-ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SARS-CoV-2 nsp10 also known as non-structural protein 10 serves as a cofactor essential for the viral life cycle. It is about 15 kDa in mass and is expressed within infected host cells where the virus replicates. Mechanically nsp10 facilitates the formation of nsp10-nsp14 and nsp10-nsp16 complexes enhancing the exonuclease and methyltransferase activities of its partners. This function supports the viral RNA proofreading and mRNA cap methylation processes contributing to the clinical pathogenicity of the virus.
Biological function summary
Nsp10 plays a role in stabilizing nsp14 and nsp16 to help them perform their enzymatic functions effectively. These interactions involve complex formations required for maintaining the integrity of viral RNA synthesis. By coordinating with nsp14 nsp10 ensures the accurate proofreading of RNA to protect against mutations. In association with nsp16 it assists in mRNA cap methylation which is pivotal for evading host immune responses.
Pathways
The interaction between nsp10 and its partner proteins integrate it into important viral replication and host immune evasion pathways. These pathways are critical for the SARS-CoV-2 lifecycle facilitating replication and transcription mechanisms. Through its role with nsp14 nsp10 contributes to the exonuclease pathway important for RNA error correction. Its association with nsp16 is significant in the methylation pathway preventing premature degradation of viral mRNA by the host cell’s immune system.
Nsp10 directly influences the progression of COVID-19 the disease caused by SARS-CoV-2. Its interactions with nsp14 and nsp16 affect the virus's ability to replicate efficiently and avoid detection by host defenses which can influence the severity of the disease. Understanding the dynamics of nsp10's interactions offers potential therapeutic targets for treating COVID-19 and managing the viral replication process to curb the disease's impact.