Abcam, ab287837, Pyruvate Dehydrogenase Activity Assay Kit

Size: 96Test
Pyruvate Dehydrogenase Activity Assay Kit (ab287837) relies on the action of PDH on pyruvate, with subsequent enzyme reactions that produce a colored product. Readout on any colorimetric (450 nm) plate reader.
Key facts
Detection method:Colorimetric,
Sample types:Adherent cells, Cell culture media, Purified mitochondria, Tissue,
Assay type:Enzyme activity,
Sensitivity:< 0.1 mU/well,
Assay Platform:Microplate (12 x 8 well strips)

Product details:
Pyruvate Dehydrogenase Activity Assay Kit ab287837 provides a quick and easy way to monitor PDH activity. In the assay, PDH converts pyruvate into an intermediate, which reduces the developer to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect pyruvate dehydrogenase activity lower than 0.1 mU in a variety of samples.
Other Notes
This product is manufactured by BioVision, an Abcam company and was previously called K679 Pyruvate Dehydrogenase (PDH) Activity Colorimetric Assay Kit. K679-100 is the same size as the 96 test size of ab287837.
Pyruvate Dehydrogenase (PDH) has a vital role in carbohydrate metabolism. It forms a well-characterized enzyme complex with dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3). PDH converts pyruvate into acetyl-CoA in the presence of NAD and CoA, and links glycolysis to the citric acid cycle. PDH activity is inhibited by high intracellular ratios of ATP/ADP, NADH/NAD or Acetyl-CoA/CoA. In humans, PDH deficiency reduces mitochondrial function and is linked to neurodegenerative diseases. PDH deficiency is X-linked; it results in 2 forms of abnormality: a metabolic form (lactic acidosis) and a neurological form (seizure and/or neuropathological spasm). Recent studies show that PDH is a target of oncogene-induced senescence; activation of PDH enhances pyruvate utilization and increases respiration and redox stress.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C