Abcam, ab287858, Live/Dead Cell Viability Assay Kit

Size: 96Test
Live/Dead Cell Viability Assay Kit ab287858 provides an easy to use method that is based on the simultaneous determination of live and dead cells using two different fluorescent dyes. We recommend Live-Dead assay ab115347 instead of this kit. It contains the same Ethidium homo-dimer and Calcein AM dyes as this kit.
Key facts
Detection method:Flow cytometry-fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay type:Quantitative,
Assay Platform:Flow cytometer, Fluorescence microscope

Product details:
This Live/Dead Cell Viability Assay Kit, provides a two-color fluorescence method that is based on the simultaneous determination of live and dead cells using two different dyes. Live cell dye easily penetrates intact, live cells and intracellular esterase hydrolyzes the dye to produce a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm which can be measured at Ex/Em = 485/530 nm. Dead cell dye enters damaged cell membranes and undergoes a 40-fold enhancement of fluorescence upon binding to nucleic acid, thereby producing a bright red fluorescence (Ex/Em = 495/635 nm) in dead cells. This assay kit provides an easy-to-use, non-radioactive, histological and FACS-based method for measuring cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.
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The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C