Abcam, ab288567, Anti-RAB35 antibody [MJF-R34-25]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal PON1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:MJF-R34-25,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RAB35 is a small GTPase belonging to the Ras superfamily and is sometimes referred to as 'Rab GTP-binding protein' and 'RAB35 member RAS oncogene family'. The protein plays an important mechanical role in endocytic recycling and intracellular trafficking. It acts by cycling between an active GTP-bound and an inactive GDP-bound state which allows it to coordinate intracellular transport processes. RAB35 is a 23 kDa protein and is expressed in various tissues with high levels observed in the brain and immune cells.
Biological function summary
RAB35 is necessary for maintaining endosomal sorting and recycling by facilitating the formation of tubular recycling compartments. It functions as part of a complex with other molecules like the exocyst complex which is needed for exocytosis. Through these interactions RAB35 ensures the proper distribution of proteins and lipids at the plasma membrane supporting cellular activities such as cell migration and synaptic function in neurons.
Pathways
RAB35 has significant roles in both the endocytic and exocytic pathways operating alongside proteins such as ARF6 and EPIST. In the endocytic pathway RAB35 is involved in the recycling of endosomes ensuring that receptors and other proteins get recycled back to the cell surface. In the exocytic pathway it aids the exocyst complex which is important for vesicle tethering and fusion at the plasma membrane a process vital for cellular secretion and communication.
RAB35 relates to conditions such as cancer and neurological disorders. Dysregulation of RAB35 has been observed in several types of cancer where it may influence cell proliferation and invasion. RAB35 is also implicated in neurological disorders possibly due to its role in maintaining synaptic function and neuronal communication. In these contexts it has potential interactions with proteins like RAS oncogene family members and other molecules involved in cell growth and differentiation.