Abcam, ab288697, Human SLC7A5 knockout HEK-293 cell line

Size: 1000000Cells / vial / 2 x 1000000Cells / vial
SLC5A2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 91 bp and 86 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 91 bp and 86 bp deletion in exon 2

Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC5A2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SLC7A5/LAT1 or LAT1 transporter also known as the large neutral amino acid transporter small subunit 1 plays an important role in facilitating the transport of large neutral amino acids across cell membranes. LAT1 is a heterodimer with a heavy chain known usually as SLC3A2 forming a functional transporter which is around 40-50 kDa in mass. LAT1 is highly expressed in tissues with high metabolic rates like the brain placenta and some cancer cells making it important for nutrient transport in these areas.
Biological function summary
The LAT1 transporter mediates the uptake of essential amino acids which are critical for protein synthesis and cellular metabolism. It functions as part of the LAT1-SLC3A2 heterodimer complex ensuring effective transport across the blood-brain barrier and into other metabolically active tissues. This activity supports cellular growth and proliferation underlining its importance in rapidly dividing cells such as in cancer.
Pathways
LAT1 plays a critical role in the mTOR signaling pathway which is central to regulating cell growth and metabolism in response to nutrient availability. LAT1's transport function influences the activation of mTORC1 by ensuring a steady supply of leucine an amino acid that activates this complex. LAT1 is also integral to the cross-pathway interaction with the insulin signaling pathway enhancing nutrient-dependent growth signals in conjunction with other transporters.
Abnormal LAT1 function links closely with cancer and neurological disorders. Its overexpression often marks various tumors where it supports cancer cell survival by sustaining amino acid supply often in conjunction with increased SLC3A2 expression. In neurological contexts LAT1 impairments can contribute to disorders involving neurotransmitter imbalances as it participates in amino acid transport critical for neurotransmitter synthesis.