Abcam, ab289622, SARS-CoV-2 Spike S1+S2 (mut L18F+T20N+P26S+D138Y+R190S+K417T+E484K+N501Y+D614G+H655Y+T1027I)

Size: 100µg
SARS-CoV-2 Spike S1+S2 (mut L18F+T20N+P26S+D138Y+R190S+K417T+E484K+N501Y+D614G+H655Y+T1027I) is a SARS-CoV-2 Fragment protein, in the 1 to 1213 aa range, expressed in Baculovirus infected insect cells, with >85%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>85% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:Baculovirus infected insect cells,
Tags:His tag C-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) at 2 μg/mL (100 μL/well) can bind ab289622, the EC50 of ab289622 is 100-600 ng/mL.,
Accession:P0DTC2,
Animal free:No,
Carrier free:No,
Species:SARS-CoV-2,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 5% Mannitol, 5% Trehalose, 1.753% Sodium chloride, 0.242% Tris, 0.01% Tween 80

Product details:
The mutations were identified in the SARS-CoV-2 variant (known as variant P.1) which emerged in the Brazil.

Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SARS-CoV-2 Spike Ectodomain often referred to simply as the spike protein is a critical component of the virus that enables entry into host cells. This glycoprotein is about 180 kDa and is prominently expressed on the viral surface. The spike protein is marked by significant regions including the S1 and S2 subunits which facilitate the binding and fusion processes necessary for viral entry into human cells. Variants like the D614G and notable mutations such as H69 deletion or D80A affect its structure. The spike protein is central to vaccine design due to its role in mediating infection.
Biological function summary
The spike protein is critical for the interaction with the host angiotensin-converting enzyme 2 (ACE2) serving as an important component in the viral entry into human cells. It forms a trimeric complex important for mediating the fusion of the viral membrane with the host cell's membrane. Structural changes such as deletions in the HV69-70 region or mutations like D80A can impact the protein's functionality and viral virulence. Therefore understanding these mutations has implications for infection control efforts.
Pathways
The spike protein plays a central role in the viral invasion pathway that starts with the recognition of the ACE2 receptor on the host cell. This pathway integrates the spike protein's functionality with other viral and host proteins leading to cell entry and subsequent viral replication. The renin-angiotensin system (RAS) is closely related given the ACE2 receptor's involvement in this physiological pathway highlighting the importance of these interactions in both viral pathology and host response.
The spike protein is instrumental in the pathogenesis of COVID-19 and related respiratory disorders. Its interaction with ACE2 is central in determining the infection's severity and transmission potential. Additionally the spike protein's structural changes such as HV69-70 deletions or D80A mutations can alter the disease manifestation by affecting how the immune system recognizes the virus. This structural variability potentially impacts vaccine efficacy and therapeutic antibody effectiveness complicating disease management and treatment strategies.