Abcam, ab289700, Mouse VEGFB ELISA Kit

Size: 1 x 96Tests
Mouse VEGFB ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse VEGFB with a sensitivity of 39.432 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Tissue Extracts, Cell Lysate,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 39.432 pg/mL,
Range:171.875 - 11000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse VEGFB ELISA Kit (ab289700) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse VEGFB in cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse VEGFB with 39.432 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
VEGFB also known as vascular endothelial growth factor B is a member of the VEGF family. It has a molecular mass of approximately 34-35 kDa. VEGFB interacts with its receptors to promote angiogenesis increasing blood supply to tissues. Expression of VEGFB occurs in the heart skeletal muscle and various endothelial cells. It also shows expression in tissues undergoing high metabolic activities. Besides its angiogenic role VEGFB exhibits participation in metabolic regulation.
Biological function summary
VEGFB supports angiogenesis by promoting endothelial cell growth and migration. It acts as a signal protein not forming part of a larger complex. VEGFB binds to VEGFR-1 (Flt-1) receptor which is involved in transmitting the growth-promoting signals. Its function extends to the regulation of fatty acid uptake into tissues highlighting its role in lipid metabolism and energy utilization.
Pathways
The signalling activities of VEGFB are significant in angiogenesis and metabolic pathways. VEGFB interacts closely with the VEGF-A pathway which is vital for forming new blood vessels. It also shares interactive roles with similar proteins such as VEGF-C and VEGF-D contributing to various angiogenic processes and cellular growth signalling systems.
Understanding VEGFB's role is important in conditions like cardiovascular disease and metabolic syndrome. Altered VEGFB signalling may link to heart diseases due to its regulatory function in maintaining heart vasculature. VEGFB has connections with VEGFR-1 and VEGF-A indicating its potential involvement in endothelial dysfunction and highlighting its significance in therapeutic strategies targeting such diseases.