Abcam, ab29, Anti-PCNA antibody [PC10]

Size: 100µg
Anti-PCNA antibody [PC10] (ab29) is a mouse monoclonal antibody detecting PCNA in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Over 730 publications - Trusted since 1998
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:PC10,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, ICC/IF, Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
This antibody clone [PC10] is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact
orders@abcam.com
or you can find further information
Product Specifications
Anti-PCNA antibody [PC10] (ab29) is a mouse monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB in human, mouse, rat samples.
Anti-PCNA antibody [PC10] (ab29) specifically detects PCNA (UniProt ID: P12004; Molecular weight: 29kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-PCNA antibody [PC10] (ab29) has high sensitivity and specificity.
Anti-PCNA antibody [PC10] (ab29) has been cited over 736 times in peer reviewed journals and is trusted by the scientific community.
Anti-PCNA antibody [PC10] (ab29) has 72 independent reviews from customers.
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Conjugation-ready, carrier free format available for antibody clone PC10 -
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Antibody clone PC10 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, HRP, Alexa Fluor® 647, PE (
ab201672
ab201673
ab201674
ab233658

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PCNA or Proliferating Cell Nuclear Antigen functions as a sliding clamp for DNA polymerases during DNA replication. This important role enables it to coordinate the orderly duplication of the genome by increasing the processivity of DNA polymerase δ. PCNA weighs approximately 29 kDa and forms a homotrimeric ring structure. It is expressed in the nucleus of cells often found abundantly in proliferating cells. Aside from its main name some people also refer to it as PC10.
Biological function summary
This protein serves a core function in cell cycle regulation and DNA repair. PCNA acts as a scaffold for the assembly of numerous proteins involved in DNA processing forming part of the replication fork complex. It interacts with cyclins and cyclin-dependent kinases (CDKs) linking DNA synthesis to cell cycle progression. Furthermore PCNA partners with proteins involved in DNA mismatch repair base excision repair and nucleotide excision repair.
Pathways
PCNA is integral in the DNA replication and repair pathways. It closely associates with the p21 protein which regulates the cell cycle by inhibiting cyclin-CDK complexes upon DNA damage. PCNA also plays a part in the ubiquitin-proteasome pathway where its modification by ubiquitin impacts how cells respond to DNA damage. This modification recruits specific proteins for DNA repair highlighting its central role in maintaining genomic stability.
PCNA is linked to cancer and autoimmune diseases. Its overexpression is common in various cancers reflecting its role in cell proliferation and the cell cycle. PCNA antibodies are sometimes found in the serum of patients with systemic lupus erythematosus (SLE) a connection also related to alterations in the p21 protein pathway. The interaction between PCNA and disease-specific proteins emphasizes its importance in both cell proliferation and immune system disorders.