Abcam, ab290749, Anti-YTHDF1 + YTHDF3 + YTHDF2 antibody [EPR26183-69] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal YTHDF1 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF, IP, WB and reacts with Rat, Mouse, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26183-69,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Rat, Mouse,
Applications:WB, ICC/IF, Flow Cyt (Intra), IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
YTHDF1 YTHDF2 and YTHDF3 also known as YTH domain family proteins are key readers of N6-methyladenosine (m6A) RNA modifications. These proteins each weigh around 63-68 kDa and show expression across various human tissues with notable activity in the brain and liver. YTHDF proteins bind to m6A-modified RNAs facilitating subsequent RNA metabolism processes such as translation and decay. By recognizing m6A marks YTHDF1 2 and 3 influence RNA fate therefore playing an important role in post-transcriptional gene regulation.
Biological function summary
YTHDF proteins facilitate the dynamic control of mRNA stability and translation efficiency. They are known to interact with each other forming complexes that further strengthen their functional impact on RNA targets. In particular YTHDF1 enhances translation by recruiting ribosomes to m6A-modified mRNAs. YTHDF2 primarily targets mRNA for degradation therefore reducing mRNA stability. Meanwhile YTHDF3 supports both YTHDF1-mediated translation and YTHDF2-mediated degradation acting as a versatile mediator in RNA regulation.
Pathways
These proteins play important roles in cellular homeostasis and stress responses. The m6A modification pathway involves collaboration with proteins such as METTL3 and FTO which add and remove m6A marks respectively. The interaction of YTHDF proteins within this pathway allows cells to quickly respond to stimuli by adjusting protein production levels. Their functions align with signaling pathways like the mTOR pathway where they influence translation rates in response to nutrient availability.
YTHDF proteins are linked to cancers and neurological conditions. Dysregulation of these proteins associates with tumorigenesis particularly through interactions with oncogenic proteins such as c-Myc. Abnormal YTHDF function also connects to neurodegenerative disorders where alterations in mRNA metabolism lead to neural dysfunction. The ability of YTHDF proteins to bind specific m6A-modified mRNAs makes them targets for therapeutic intervention in these diseases.