Abcam, ab300060, Anti-PCGF6 antibody [EPR26301-72] (BSA and Azide free)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PCGF6 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), IP, ICC/IF and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26301-72,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, WB, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PCGF6 also known as Polycomb group RING finger protein 6 plays a role in regulating gene expression. This protein with a molecular mass of about 36 kDa is part of the Polycomb Group (PcG) proteins that epigenetically silence genes preventing their expression. Scientists have observed PCGF6 expression in embryonic stem cells as well as in various tissues hinting at its broad potential impact on cellular processes. The protein contains a RING finger motif which suggests it functions as an E3 ubiquitin ligase tagging other proteins for modification or degradation.
Biological function summary
PCGF6 participates in transcriptional repression through its involvement in the non-canonical Polycomb Repressive Complex 1 (PRC1). The protein acts by aiding the assembly of PRC1 alongside other constituents such as RING1B to modify histones and maintain stem cell characteristics. This activity is important for cell differentiation and maintaining the balance between pluripotency and lineage specification during development. Disruptions in PCGF6 function may influence epigenetic landscapes significantly thereby impacting cellular identity and behavior.
Pathways
PCGF6 integrates its activity with key biological systems such as the Wnt signaling and the polycomb repression pathways. Within these pathways PCGF6 interacts with core PRC1 components like RING1B to affect gene silencing and chromatin remodeling. These interactions allow the protein to serve as a critical node in controlling genetic hierarchies that regulate embryogenesis and other developmental processes. The pathway network involving PCGF6 and PRC1 provides essential insights into the fine-tuning of gene expressions needed for cellular differentiation.
Aberrations in PCGF6 expression or function have connections to cancer and developmental disorders. Altered PCGF6 activity can lead to dysregulated expression of genes that are normally repressed fostering oncogenic or detrimental developmental outcomes. In the context of cancer its interaction with components such as BMI1 helps to drive tumor progression by supporting unchecked cell proliferation. Furthermore understanding these interactions highlights potential therapeutic avenues by targeting the components influenced by PCGF6 offering opportunities for better management of these diseases.