Abcam, ab300086, Anti-APOA1 antibody [EPR25446-9] (BSA and Azide free)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Apolipoprotein A I antibody. Carrier free. Suitable for WB and reacts with Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25446-9,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
APOA1 also known as Apolipoprotein A-I plays an important role in lipid metabolism. Mechanically APOA1 acts as the major protein component of high-density lipoprotein (HDL) in plasma. It facilitates cholesterol efflux from tissues to the liver for excretion. With a molecular weight of approximately 28 kDa APOA1 is expressed mainly in the liver and small intestine. This protein is also involved in lecithin-cholesterol acyltransferase (LCAT) activation which is important for the maturation of HDL particles.
Biological function summary
APOA1 is instrumental in reverse cholesterol transport a process that removes excess cholesterol from cells and prevents lipid accumulation. APOA1 is part of the HDL complex and it directly interacts with various lipid molecules. It contributes to lipid homeostasis and has antioxidant properties reducing oxidative stress. APOA1 also has anti-inflammatory effects which play a role in maintaining cardiovascular health.
Pathways
The reverse cholesterol transport pathway is highly reliant on APOA1 functionality supporting cholesterol movement out of cells. In this pathway APOA1 interacts with ATP-binding cassette transporters like ABCA1 and ABCG1 which aid in transferring cholesterol to HDL particles. Additionally APOA1 is associated with the HDL metabolism pathway where it collaborates with other proteins such as Apolipoprotein A-II (APOA2) and Lecithin-Cholesterol Acyltransferase (LCAT) facilitating the conversion of nascent HDL into mature HDL.
APOA1 has significant implications in cardiovascular diseases and dyslipidemia. Deficiency or dysfunction of APOA1 can lead to lowered HDL levels which correlates with an increased risk of atherosclerosis and coronary artery disease. Furthermore research shows a connection between APOA1 and amyloid-related diseases; abnormal processing or accumulation of apolipoprotein A-I can contribute to amyloidosis. The interactions with proteins like Apolipoprotein E (APOE) are noted as APOE plays a role in lipid metabolism and is implicated in neurodegenerative disorders such as Alzheimer's disease.