Abcam, ab300109, Anti-pan PDE4 antibody [EPR25202-101] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PDE4D antibody. Carrier free. Suitable for WB, Dot, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25202-101,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:WB, Flow Cyt (Intra), Dot, ICC/IF, IHC-P, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PDE4 or phosphodiesterase 4 is an enzyme that specifically hydrolyzes cyclic adenosine monophosphate (cAMP) into AMP which regulates intracellular levels of cAMP. PDE4 subfamily includes four isoforms: PDE4A PDE4B PDE4C and PDE4D each with unique expression patterns and roles. The approximate molecular weight of PDE4 enzymes varies depending on isoform but typically around 80-100 kDa. This enzyme is widely distributed in the body with expression found primarily in the brain heart lungs and immune cells where it modulates cellular responses to hormonal signals.
Biological function summary
PDE4 plays an important role in modulating inflammatory responses and immune cell activation due to its control over cAMP levels. By degrading cAMP PDE4 impacts the signaling pathways that govern inflammation and immune responses. This enzyme is often found in complex with other proteins that help localize and regulate its activity within cells. It plays a significant role in neuron communication and neuroplasticity affecting learning and memory processes.
Pathways
PDE4 is integral to the cAMP signaling pathway which impacts numerous physiological processes including cardiac function and immune responses. It interacts closely with protein kinase A (PKA) as its downstream effector thereby influencing PKA-dependent cellular responses. PDE4 also interacts within the mitogen-activated protein kinase (MAPK) pathway linking it to cell growth and differentiation responses which are important for various cellular processes.
PDE4 is strongly implicated in inflammatory and neuropsychiatric conditions. Overactivity or dysregulation of PDE4 can lead to disorders such as asthma and chronic obstructive pulmonary disease (COPD) due to its role in inflammation. It is also connected to major depressive disorder where altered cAMP signaling plays a part in pathophysiology. Inflammatory responses are linked to proteins such as tumor necrosis factor-alpha (TNF-alpha) and interleukins where PDE4 modulation can influence these pathways affecting disease development and progression.