Abcam, ab300457, Anti-TMEM175 antibody [EPR24415-47]

Size: 20µL / 100µL / 1mL
Anti-TMEM175 antibody [EPR24415-47] (ab300457) is a rabbit monoclonal antibody detecting TMEM175 in Western Blot, IHC-P . Suitable for Human . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24415-47,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-TMEM175 antibody [EPR24415-47] (ab300457) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
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Specificity confirmed
The specificity of Anti-TMEM175 antibody [EPR24415-47] (ab300457) has been confirmed by Western blot testing in TMEM175 Knockout HeLa cell line,
ab265571
Other related products
We have a range of other formats of antibody clone [EPR24415-47] also available for your convenience: ab300457, Carrier free -
ab300458
ab309572
, PE -
ab318400
, APC -
ab318503
, Alexa Fluor® 488 -
ab318606
, Alexa Fluor® 647 -
ab318709
, Alexa Fluor® 594 -
ab318812
, Alexa Fluor® 555 -
ab318912
, Alexa Fluor® 750 -
ab321547
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TMEM175 also known as Transmembrane Protein 175 is a channel protein with dual function as a lysosomal potassium channel. It has an approximate mass of 66 kDa. TMEM175 plays a role in ion homeostasis and is expressed mainly in tissues with high levels of lysosomes including the brain and the immune system. This protein aids in the maintenance of the lysosomal environment important for cellular activities.
Biological function summary
This lysosomal protein contributes to the maintenance of ion stability within lysosomes an important factor in lysosomal degradation and overall cellular clearance processes. TMEM175 is an integral component of the lysosomal membrane and participates in ensuring lysosomal pH stability therefore influencing autophagic activity. TMEM175 does not participate in known protein complexes but it is pivotal for preserving lysosomal integrity.
Pathways
TMEM175 actively engages in the lysosomal degradation pathway impacting cellular waste processing. TMEM175 influences endolysosomal trafficking and autophagy which are essential for metabolite recycling. It works alongside proteins like ATPase and lysosomal enzyme hydrolases by controlling ion concentrations which can influence other lysosomal functions and pathways.
TMEM175 has a significant connection with neurodegenerative conditions such as Parkinson's disease. Mutation or dysfunction in TMEM175 impacts lysosomal function linked to protein aggregation often involving alpha-synuclein which serves as a hallmark for such neurodegenerative diseases. Additionally TMEM175 is implicated in lysosomal storage disorders where impaired lysosomal function results in cellular accumulation of substances due to ineffective degradation.