Abcam, ab300507, Anti-Sodium/potassium-transporting ATPase (pan alpha subunit) antibody [EPR25414-249]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal alpha 1 Sodium Potassium ATPase antibody. Suitable for WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25414-249,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:ICC/IF, IHC-P, IHC-Fr, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The sodium/potassium-transporting ATPase also known as Na+/K+ ATPase or the pan alpha subunit functions as an essential pump that exchanges three sodium ions out of the cell for two potassium ions into the cell using ATP as the energy source. This process maintains the electrochemical gradients of sodium and potassium across the plasma membrane. The ATPase has a molecular mass of approximately 112 kDa and consists of multiple isoforms including ATP1A1 ATP1A2 ATP1A3 and ATP1A4 expressed in various tissues such as the brain kidney and heart.
Biological function summary
The Na+/K+ ATPase is part of a larger complex that influences numerous cellular activities including cell volume regulation and secondary active transport. It helps maintain the resting potential of cells and facilitates transport of nutrients and other molecules across cell membranes. This pump’s activity is critical for nerve impulse transmission muscle contraction and kidney function tying it to several essential physiological processes.
Pathways
The Na+/K+ ATPase plays a role in key pathways like the cardiac contractility pathway and the neural activity pathway. In the cardiac contractility pathway it influences heart muscle contraction and is functionally related to proteins such as phospholamban and SERCA. In neural activity it helps generate action potentials and is closely related to the function of voltage-gated sodium channels.
Mutations or malfunctions in the Na+/K+ ATPase can relate to conditions like familial hemiplegic migraine and cardiac arrhythmias. In familial hemiplegic migraine changes in ATP1A2 have been implicated influencing ion homeostasis in neurons. In cardiac arrhythmias ATP1A1 has connections to abnormal ion gradients affecting the rhythm of heartbeats and linking it to other proteins involved in cardiac excitability.