Abcam, ab300509, Anti-ALDH1L1+ALDH1L2 antibody [EPR25443-54] - Astrocyte Marker

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal ALDH1L1 antibody. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, Dot, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Mouse samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25443-54,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Mouse, Human,
Applications:Dot, IHC-Fr, ICC/IF, IHC-P, Flow Cyt (Intra), IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ALDH1L1 and ALDH1L2 also known as 10-formyltetrahydrofolate dehydrogenase isoform 1 and 2 are enzymes involved in folate metabolism. ALDH1L1 has a molecular mass of approximately 100 kDa and is prominently expressed in the liver and brain. ALDH1L2 is found mainly in the mitochondria across various tissues. Both enzymes catalyze the conversion of 10-formyltetrahydrofolate to tetrahydrofolate and carbon dioxide playing an important role in cellular metabolism.
Biological function summary
The ALDH1L1 and ALDH1L2 participate in the regulation of one-carbon metabolism which is essential for nucleotide biosynthesis and amino acid interconversion. ALDH1L1 functions as part of a complex with other enzymes involved in folate metabolism facilitating the transfer of one-carbon units. These enzymes assist in maintaining proper cellular levels of folate derivatives fundamental for methylation processes and DNA synthesis.
Pathways
ALDH1L1 and ALDH1L2 contribute to the folate cycle and the related methionine cycle. They participate alongside proteins such as methylenetetrahydrofolate reductase (MTHFR) and methionine synthase both of which play roles in homocysteine regulation. Proper functioning of these pathways is necessary for efficient methyl group transfer important for numerous methylation reactions within cells.
Impaired function of ALDH1L1 and ALDH1L2 is associated with metabolic disorders including hyperhomocysteinemia and certain types of cancer. Abnormal activity of these enzymes can cause disruptions in folate metabolism leading to elevated homocysteine levels which in turn are linked to cardiovascular diseases. Additionally studies suggest that alterations in folate pathway enzymes including MTHFR might influence cancer progression by affecting DNA methylation status and gene expression.