Abcam, ab300564, Anti-ASIC1 antibody [EPR25411-161] (BSA and Azide free)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ASIC1 antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Mouse, Rat, Transfected cell line - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25411-161,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat, Mouse,
Applications:IP, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ASIC1 or acid-sensing ion channel 1 is also known as ASIC or ACCN2. It is part of a group of proton-gated cation channels. ASIC1 has a molecular mass of approximately 60 kDa. This ion channel plays a role in the detection of acidosis where it mediates proton influx across cell membranes affecting changes in membrane potential. ASIC1 is expressed in neurons of the central and peripheral nervous systems with particular abundance in the brain. Its localization to regions such as the amygdala suggests a possible role in processing emotions and pain perception.
Biological function summary
This ion channel aids in neuronal response to pH drops serving as a sensor for acidification. The ASIC1 subunit can form homotrimers or heterotrimers with other ASIC subunits which impacts the ion conductance properties. This protein's activity affects synaptic plasticity and is involved in both short-term and long-term modulation of neuronal circuits. Changes in its activity can influence neurotransmission and has implications in neurological functionality.
Pathways
ASIC1 is part of the neurophysiological signaling pathways affecting synaptic transmission. It interacts with other proteins like ASIC2 and ASIC3 being implicated in the modulation of sensory perception and pain. Involvement in pathways like the pain-transducing signaling pathway places ASIC1 in a context where it can regulate nociceptive signals. It participates in ion flux alterations that contribute to neuronal excitability and synaptic strength.
ASIC1 has connections to neurodegenerative conditions and pain syndromes. It is implicated in the pathophysiology of disorders such as ischemia where acidosis occurs during stress and tissue damage and chronic pain conditions. ASIC1 interacts with proteins such as the NMDA receptor in the context of these diseases suggesting a role in excitotoxicity. Dysfunction or altered regulation of ASIC1 may exacerbate neurodegeneration or chronic pain symptoms highlighting its potential as a therapeutic target.