Abcam, ab300626, Anti-Phospho - (Ser/Thr) Phe antibody [EPR26858-4] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Phospho - (Ser/Thr) Phe antibody. Carrier free. Suitable for ELISA, WB, Dot, IHC-P, ICC/IF, Flow Cyt (Intra), IP and reacts with Modified Amino Acid samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26858-4,
Isotype:IgG,
Carrier free:Yes,
Applications:ICC/IF, Flow Cyt (Intra), Dot, ELISA, IHC-P, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:AKT and several other basophilic protein kinases, for example, PKCa, PKCg, and PKCd, select substrates with Phe at +1 position (PMID: 10945990). ab300626 specifically detects phospho-serine or threonine with Phe at +1 position.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phospho-(Ser/Thr) Phe commonly referenced as phosphorylated serine or threonine and sometimes noted as phospho plays an important role mechanically in signal transduction processes by modifying protein function through phosphorylation. It is not a singular protein but rather refers to a specific post-translational modification wherein a phosphate group is added to serine or threonine residues of proteins. Given its nature it does not have a fixed molecular mass. This phosphorylation occurs in various cell types including muscle brain and liver cells as the proteins involved are ubiquitous components of signaling pathways across different tissues.
Biological function summary
The addition of a phosphate group to serine or threonine residues alters protein conformation affecting enzyme activity protein-protein interactions and cellular localization. This modification participates in protein complexes enabling the proteins to act as switches in anabolic and catabolic processes. In many cellular tasks phospho-antibodies detect these modified residues allowing researchers to delineate protein activity states in cellular signaling.
Pathways
Phospho-(Ser/Thr) Phe holds significant importance in the MAPK and AKT signaling pathways. Proteins such as RAF MEK and ERK in the MAPK pathway and AKT and mTOR in the AKT pathway are heavily regulated by serine/threonine phosphorylation. This regulation ensures proper cellular responses to stimuli like growth factors and stress linking extracellular signals to cellular responses.
Dysregulation of phospho-(Ser/Thr) Phe modifications can lead to conditions like cancer and neurodegenerative diseases. In cancer for example aberrant phosphorylation in the MAPK pathway involving proteins such as BRAF and KRAS contributes to uncontrolled cell proliferation. Similarly in neurodegenerative diseases improper phosphorylation of proteins like tau can result in toxicity leading to a cascade of cellular dysfunction and disease progression.