Abcam, ab301148, Human PER2 knockout A549 cell line

Size: 1000000Cells / vial / 2 x 1000000Cells / vial
PER2 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,
Disease:Carcinoma

Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PER2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PER2 protein short for Period Circadian Regulator 2 is an important component of the molecular circadian clock. Alternate names for this protein include PER 2 and per2. This protein has a mass of about 136 kDa and is expressed in various tissues including the brain and liver. PER2 mechanically functions through its role as a transcriptional regulator influencing the expression of genes involved in maintaining circadian rhythm. It forms complexes with other circadian proteins to help regulate these processes.
Biological function summary
The PER2 protein plays an important role in the regulation of genes that control the body's circadian rhythms. It participates in the negative feedback loop of the circadian clock where it is part of a complex with other proteins including CRY1 and CRY2. This complex inhibits its own transcription by repressing the activity of CLOCK and BMAL1 which are core circadian transcription factors. This process results in the oscillations that define circadian rhythms affecting sleep-wake cycles hormone release and various metabolic processes.
Pathways
PER2 is integral to the circadian rhythm pathway and is involved in the regulation of several physiological processes. PER2 along with other proteins like PER1 interacts with the CLOCK-BMAL1 complex which is important in setting and maintaining the circadian rhythms. The interplay of these proteins governs the transcriptional-translational feedback loops that are the foundation of circadian regulation. This pathway is critical for synchronizing the internal clock with the external environment ensuring that biological processes occur at the appropriate times.
PER2 has been linked to sleep disorders such as Familial Advanced Sleep Phase Syndrome (FASPS) and certain mood disorders. Mutations or dysregulation in the PER2 gene may disrupt normal circadian rhythms leading to these conditions. Additionally PER2 has shown connections with cancer where its expression levels can influence tumor progression particularly through interactions with proteins involved in cell cycle regulation. Understanding the precise role of PER2 in these diseases might provide insights for potential therapeutic targets.