Abcam, ab301181, Human PRKD2 knockout A549 cell line

Size: 1000000Cells / vial / 2 x 1000000Cells / vial
PRKD2 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,Western blot,
Disease:Carcinoma

Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PRKD2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PKD2 also known as Polycystin-2 or TRPP2 is a calcium-permeable cation channel with an approximate mass of 97 kDa. It is part of the transient receptor potential polycystic (TRPP) subfamily. PKD2 primarily localizes to the endoplasmic reticulum membrane but also found at plasma membranes and cilia. This protein is widely expressed in different tissues including kidney heart brain and muscles reflecting its diverse role in cellular physiology.
Biological function summary
PKD2 plays a significant role in maintaining calcium ion homeostasis and cellular signal transduction. PKD2 functions together with PKD1 forming a complex that is essential in mechanosensation and ciliary signaling. When part of this complex PKD2 helps regulate cellular responses to mechanical stimuli which are important in tissue architecture and integrity. Furthermore PKD2 influences various cellular processes by modulating ion fluxes and intracellular signaling pathways.
Pathways
PKD2 is involved in the polycystic kidney disease pathway and the Wnt signaling pathway. These pathways are integral to cell proliferation differentiation and tissue maintenance. In the polycystic kidney disease pathway PKD2 interacts with PKD1 affecting cellular processes related to cyst formation. Additionally PKD2's role in the Wnt pathway which is vital for cellular communication and development highlights its cooperation with other signaling proteins like beta-catenin linking it to cellular growth and morphology.
Mutations in the PKD2 gene are directly linked to autosomal dominant polycystic kidney disease (ADPKD) which leads to the formation of multiple cysts in the kidneys disrupting normal function. PKD2 protein's interaction with PKD1 is critical in ADPKD pathogenesis indicating that PKD1 and PKD2 mutations cumulatively affect disease severity. Additionally aberrant PKD2 expression has an association with cardiac conditions such as cardiac hypertrophy reflecting its importance in maintaining cardiovascular health.