Abcam, ab302515, Anti-HuD antibody [EPR26472-54] (BSA and Azide free)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HuD antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26472-54,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, WB, Flow Cyt (Intra), ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The HuD protein also known as ELAV-like protein 4 is a neuronal RNA-binding protein with a molecular mass of approximately 40 kDa. It belongs to the ELAV family of proteins which is well-studied for its involvement in mRNA processing. HuD expresses predominantly in the nervous system where it is present in neurons and developing neural tissue. The protein localizes to the cytoplasm where it binds to mRNA influencing the stability and translation of target mRNAs.
Biological function summary
HuD is essential in regulating neuronal differentiation and function. The protein binds to adenylate-uridylate-rich elements (AREs) within the 3' untranslated regions (UTR) of specific mRNAs. It stabilizes these mRNAs contributing to the post-transcriptional regulation of genes linked to synaptic plasticity and neuronal development. HuD does not typically form part of a larger complex but interacts with other RNA-binding proteins to exert its regulatory effects.
Pathways
HuD plays a significant role in neuronal development and synaptic plasticity. It involves in pathways like the MAPK signaling pathway which is critical for cell differentiation and the mTOR pathway which modulates protein synthesis. In these pathways HuD interacts with proteins such as CPEB and FMRP which also have functions related to RNA regulation and neuronal growth.
HuD's function impacts neurological conditions such as Alzheimer's disease and certain types of cancer. Changes in the expression or activity of HuD can influence the progression of Alzheimer's disease by affecting mRNA stability of genes associated with neural health. Additionally abnormal HuD expression links to neuroblastoma a cancer showing high HuD levels promoting uncontrolled cell growth. In these diseases HuD interacts with tau protein and MYCN respectively contributing to disease mechanisms.