Abcam, ab302612, Anti-SUR1 antibody [EPR25761-44] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SUR1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25761-44,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Rat,
Applications:ICC/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SUR1 protein also known as ABCC8 is an important subunit of ATP-sensitive potassium (K_ATP) channels. This protein has a molecular mass of approximately 158 kDa. SUR1 is expressed mainly in pancreatic beta cells although one can also find it in neurons and cardiac tissue. It plays an essential role in regulating the membrane potential and in turn controlling insulin secretion. SUR1 is a member of the ATP-binding cassette (ABC) transporter family which contributes to its ability to influence cellular responses to changes in metabolic states.
Biological function summary
SUR1 binds with the Kir6.2 channel subunits to form functional K_ATP channel complexes. These channels respond to intracellular ATP and ADP levels providing a critical link between cellular energy metabolism and electric cell function. In pancreatic beta cells the opening and closing of these channels trigger the release of insulin in response to blood glucose levels. In the brain the channels modulate neuronal excitability and play a role in neuroprotection. The coupling with Kir6.2 emphasizes the role of the SUR1-Kir6.2 complex in many cellular processes.
Pathways
The K_ATP channels containing SUR1 participate in the insulin secretion pathway and the cellular response to hypoxia. The insulin secretion pathway is important for glucose homeostasis where SUR1 interacts with proteins like Kir6.2 directly linking glucose metabolism to insulin release. In the cellular response to hypoxia SUR1 influences neuronal survival by modifying cell membrane potential regulating excitability and managing cellular energy usage in low oxygen conditions. These pathways demonstrate the importance of SUR1 in maintaining energy balance within cells and across organs.
SUR1 has a significant role in conditions such as congenital hyperinsulinism and neonatal diabetes. Mutations in the SUR1 gene (ABCC8) can lead to deregulation of insulin secretion causing the overproduction of insulin seen in congenital hyperinsulinism. Similarly other mutations might result in impaired insulin release contributing to neonatal diabetes. Additionally SUR1 interacts with Kir6.2 during these disorders and this interaction becomes a target for therapeutic strategies aimed at either activating or inhibiting K_ATP channels as needed to restore balanced insulin levels.