Abcam, ab302616, Anti-XPG antibody [EPR25351-5] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal XPG antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR25351-5,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
XPG also known as ERCC5 is an important player in DNA repair mechanisms specifically involved in nucleotide excision repair (NER). It is an endonuclease meaning it cuts DNA at specific sites to help remove damaged DNA. XPG is known to have a molecular weight of approximately 133 kDa. The protein is expressed in a wide array of human tissues making its function important in maintaining genomic stability across different cell types.
Biological function summary
XPG contributes to the repair of bulky DNA lesions such as those caused by UV light. This protein functions as part of the NER complex where it performs a critical incision on the DNA strand undergoing repair. In this complex XPG works alongside other proteins like XPA and RPA coordinating the removal and repair of damaged nucleotides. Its activity is essential in correcting DNA damage and maintaining cellular health protecting against mutations that might occur from environmental damage.
Pathways
XPG plays integral roles in the nucleotide excision repair pathway. This pathway is significant for correcting DNA damage induced by ultraviolet radiation and chemical agents. XPG works closely with protein ERCC1-XPF to facilitate DNA repair highlighting its collaborative function. Moreover XPG involvement extends to the base excision repair pathway further emphasizing its importance in maintaining DNA integrity.
XPG mutations are associated with disorders such as Xeroderma Pigmentosum (XP) and Cockayne Syndrome (CS). These conditions result from impaired DNA repair due to a dysfunctional NER pathway. In XP for example individuals become highly sensitive to UV exposure leading to increased cancer risk while CS leads to neurological and developmental deficits. XPG's interaction with the protein CSA becomes particularly relevant in Cockayne Syndrome indicating a vital connection in its pathology.