Abcam, ab302715, Anti-SPHK1 antibody [EPR24511-37] (BSA and Azide free)

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SPHK1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR24511-37,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SPHK1 also known as sphingosine kinase 1 is an enzyme that catalyzes the phosphorylation of sphingosine to produce sphingosine-1-phosphate (S1P). This target has a molecular mass of approximately 47 kDa. SPHK1 is expressed in various tissues including the brain spleen heart and lungs. Its activity plays a role in cell signaling processes that are essential for cellular responses to external stimuli.
Biological function summary
Within the cell SPHK1 participates in regulating cell survival proliferation and inflammation. It is part of the sphingolipid metabolic pathway which is not a traditional protein complex but more of a metabolic sequence. SPHK1 converts sphingosine a lipid signaling molecule into S1P which acts as a ligand for G-protein-coupled receptors. Through this conversion SPHK1 influences diverse cellular functions.
Pathways
This protein actively participates in the sphingolipid signaling pathway and the apoptosis pathway. In sphingolipid signaling S1P acts alongside ceramide and other sphingolipids to modulate cell response. SPHK1's interplay with the ceramide-sphingosine-S1P rheostat can determine cell fate by tipping the balance towards survival or apoptosis. In the apoptosis pathway SPHK1 serves as a regulator of cell death with its function connected to proteins such as Bcl-2 and Bax which are primary apoptosis regulators.
Abnormal expression of SPHK1 has links to cancer and cardiovascular diseases. In cancer overexpression of SPHK1 contributes to enhanced survival and proliferation of tumor cells with a noted interaction with the protein EGFR which is often upregulated in tumors. In cardiovascular diseases dysregulated SPHK1-S1P signaling can impact vascular integrity and inflammation influencing the progression of atherosclerosis with involvement of other inflammatory proteins like NF-kB.