Abcam, ab302717, Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Ryanodine receptor 2/RYR-2 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Mouse, Rat, Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26288-70,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:IP, WB, IHC-Fr, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ryanodine receptor 2 (RYR-2) also known as cardiac ryanodine receptor is an intracellular calcium release channel that plays an important role in cardiac muscle contraction. This large protein has a mass of about 564 kDa and is mainly expressed in cardiac muscle tissue. Mechanically RYR-2 regulates the release of calcium ions from the sarcoplasmic reticulum into the cytosol. This calcium release occurs through the calcium-induced calcium release mechanism which is important in excitation-contraction coupling in cardiac muscle cells.
Biological function summary
RYR-2 enables the heart muscle cells to contract and relax by supplying calcium ions necessary for muscle contraction. It is part of a larger complex that includes proteins such as FK506-binding protein 12.6 (FKBP12.6) and calsequestrin which help regulate its function. Proper function of this complex is necessary for maintaining the rhythmic contraction and relaxation cycles of the heart. Any disruptions in this function can lead to irregular heartbeats.
Pathways
RYR-2 is significant in cardiac calcium signaling pathways that involve calcium-induced calcium release. Through this pathway it works closely with L-type calcium channels like Cav1.2 which are responsible for the initial influx of calcium that activates RYR-2. This cooperation allows for the amplification of calcium signals needed for strong cardiac muscle contractions. Proper signaling in these pathways is necessary for maintenance of heart rhythm and contractile strength.
RYR-2 mutations associate with several cardiac disorders including catecholaminergic polymorphic ventricular tachycardia (CPVT) and arrhythmogenic right ventricular dysplasia type 2 (ARVD2). These conditions involve irregular heartbeats often triggered by physical exercise or stress. The relationship between RYR-2 and these disorders connects through its dysfunction in calcium signaling regulation which impacts heart rhythm stability. The malfunctioning of RYR-2 also affects related proteins like FKBP12.6 contributing further to disrupted calcium homeostasis and arrhythmic events.