Abcam, ab303757, Human Pro-MCH ELISA Kit

Size: 1 x 96Tests
Human Pro-MCH ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Pro-MCH with a sensitivity of 100.411 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Tissue Extracts,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 100.411 pg/mL,
Range:187.5 - 12000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Pro-MCH SimpleStep ELISA® kit (ab303757) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Pro-MCH protein in tissue extracts. Quantitate Human Pro-MCH with 100.411 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pro-MCH also known as pro-melanin-concentrating hormone is a precursor polypeptide that undergoes post-translational processing to produce mature melanin-concentrating hormone (MCH). The precursor weighs approximately 18 kDa before processing. Pro-MCH is expressed mainly in the hypothalamus but can also be found in other brain regions involved in regulating appetite and energy homeostasis.
Biological function summary
The pro-MCH precursor undergoes cleavage to generate MCH which plays a significant role in energy balance and feeding behavior. MCH functions are often linked to neuronal populations in the hypothalamus that regulate hunger signals and it is involved in forming neuronal circuits that influence behavior related to food intake. Although pro-MCH itself is not part of a larger complex the derived MCH interacts with its receptors to transmit signals.
Pathways
Melanin-concentrating hormone interacts with energy homeostasis and reward circuits in the brain. It is engaged in the melanocortin pathway which balances energy intake and expenditure. MCH modulates signaling by interacting with receptors such as MCHR1 and MCHR2 influencing food-seeking behavior and energy regulation processes. Additionally its regulation aligns with orexigenic (appetite-increasing) peptides such as neuropeptide Y (NPY) coordinating energy-related responses.
Aberrant MCH signaling relates to metabolic disorders such as obesity and altered response to stress. Individuals with irregular MCH signaling may exhibit increased appetite and weight gain contributing to metabolic syndrome. The interaction with MCHR1 is significant here as alterations in this receptor are associated with such metabolic changes. Furthermore pro-MCH and its derivative peptides have also been linked to mood disorders with disrupted MCH pathways influencing depression-related behaviors through interactions with proteins like corticotropin-releasing hormone (CRH).