Abcam, ab305250, Anti-SIAH1 antibody [EPR27101-45] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SIAH1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27101-45,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SIAH1 also known as Seven In Absentia Homolog 1 functions mechanically as a RING-type E3 ubiquitin-protein ligase. This protein weighs approximately 32 kDa and adds ubiquitin to target proteins tagging them for proteasomal degradation. Researchers have found SIAH1 expressed in various tissue types including brain heart and kidney. By regulating protein stability SIAH1 plays a significant role in maintaining cellular protein homeostasis.
Biological function summary
SIAH1 influences cell cycle control and apoptosis by interacting with components of protein complexes. It associates with the ubiquitin-proteasome pathway to mediate degradation of key regulatory proteins. SIAH1 helps in reducing cellular proliferation and enhances apoptotic signaling which are vital processes for maintaining balance between cell survival and death. As part of these processes studies have connected SIAH1 to modulating the fate of proteins involved in cell cycle arrest.
Pathways
Several interactions of SIAH1 contribute to signal transduction and apoptosis. One significant pathway involving SIAH1 is the p53 signaling pathway. SIAH1 regulates the degradation of proteins within this pathway including p21 and cyclin D1 which influence cellular responses to DNA damage. Additionally SIAH1 interacts with β-catenin linking it to the Wnt signaling pathway. This interaction affects the regulation of cell growth and differentiation.
SIAH1 has links to cancer and neurodegenerative diseases. In cancer altered SIAH1 expression affects tumor suppressor activity through its interaction with p53 which can lead to uncontrolled cell growth. Moreover SIAH1 connects to neurodegenerative disorders like Parkinson's disease where abnormal protein degradation contributes to neuronal death. In such contexts researchers observe altered interactions between SIAH1 and proteins like parkin a ubiquitin ligase also implicated in these diseases.