Abcam, ab30532, Anti-LDL Receptor antibody

Size: 50µg
Anti-LDL Receptor antibody (ab30532) is a rabbit polyclonal antibody detecting LDL Receptor in Western Blot, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Over 80 publications - Trusted since 2006
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Mouse Low-density lipoprotein receptor aa 450-550. The exact immunogen used to generate this antibody is proprietary information.P35951

Product details:
Product Specifications
Anti-LDL Receptor antibody (ab30532) is a rabbit polyclonal antibody and is validated for use in ICC/IF, IHC-P, WB in human, mouse, rat samples.
Anti-LDL Receptor antibody (ab30532) specifically detects Low-density lipoprotein receptor (UniProt ID: P01130; Molecular weight: 93kDa) and is sold in 50 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-LDL Receptor antibody (ab30532) has high sensitivity and specificity.
Anti-LDL Receptor antibody (ab30532) has been cited over 88 times in peer reviewed journals and is trusted by the scientific community.
Anti-LDL Receptor antibody (ab30532) has 12 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: Tris buffered saline, 40% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The LDL Receptor also called LDLR is a protein that plays an important role in the uptake and clearance of low-density lipoproteins (LDL) from the bloodstream. LDLR binds LDL particles facilitating their internalization through receptor-mediated endocytosis. This receptor is expressed mainly in the liver adrenal glands and other tissues involved in cholesterol metabolism. It has an approximate molecular weight of 160 kDa. Researchers often study LDLR using techniques like Western blotting to understand its presence and function.
Biological function summary
The LDL receptor interacts with LDL particles to regulate cholesterol levels in the body. It is part of a cell surface complex that recognizes and binds to apolipoprotein B-100 or apolipoprotein E present on LDL. This interaction initiates internalization of LDL leading to its degradation in lysosomes where cholesterol can be released and used by the cell. Mutations in the gene encoding LDLR can lead to inefficient cholesterol uptake influencing various metabolic processes.
Pathways
LDL receptor activities are integral to lipid metabolism and cholesterol homeostasis. Two important biological pathways that involve LDLR include the cholesterol biosynthesis pathway and the lipoprotein clearance pathway. Within these pathways LDLR collaborates closely with proteins like PCSK9 which modulates its expression and degradation and HMG-CoA reductase an important enzyme in cholesterol synthesis to balance cholesterol levels in the body.
Defects in the LDL receptor are strongly associated with familial hypercholesterolemia and atherosclerosis. These conditions arise from impaired clearance of LDL leading to elevated cholesterol levels which pose risks for cardiovascular diseases. LDLR dysfunctions are linked with the Protein PCSK9 whose gain-of-function mutations can exacerbate hypercholesterolemia by promoting degradation of LDLR while statins aim to increase LDLR expression to lower LDL cholesterol levels.