Abcam, ab305527, APC Anti-HDAC1 + HDAC2 antibody [EPR20327]

Size: 100µL
Rabbit Recombinant Monoclonal HDAC1 antibody - conjugated to APC.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20327,
Isotype:IgG,
Conjugation:APC,
Excitation/Emission:Ex: 650nm, Em: 660nm,
Carrier free:No,
Applications:Antibody Labelling, Target Binding AffinitySee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
conjugated primary antibody
is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the
Lightning-Link® conjugation technology
, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.
How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HDAC1 and HDAC2 also known as histone deacetylase 1 and 2 are enzymes that remove acetyl groups from histone proteins leading to chromatin condensation and transcriptional repression. HDAC1 has a molecular weight of approximately 62 kDa and is expressed in various cell types including those in the central nervous system heart and immune cells. HDAC2 shares similar expression patterns with a molecular weight around 55 kDa. Both proteins play a role in regulating gene expression by altering chromatin structure.
Biological function summary
Both HDAC1 and HDAC2 are components of large multiprotein complexes such as the Sin3 NuRD and CoREST complexes which are involved in transcriptional regulation. These complexes facilitate the deacetylation of histones transcription factors and other cellular proteins influencing cell cycle progression differentiation and development. HDAC1 and HDAC2 modulate cellular activities impacting cell proliferation and survival.
Pathways
HDAC1 and HDAC2 participate in key cellular processes particularly the cell cycle and apoptosis pathways. These enzymes closely interact with proteins like pRb and E2F influencing the transition between different cell cycle phases. HDAC1 and HDAC2 also connect with tumor suppressor protein p53 affecting apoptotic pathways. By modulating these pathways they play an essential role in maintaining cellular homeostasis.
HDAC1 and HDAC2 are pertinent to certain cancers and neurodegenerative diseases. Their aberrant activity links to cancer progression influencing genes involved in cell cycle regulation. Overexpression or dysregulation may contribute to the development of cancer types such as leukemia. In neurodegenerative diseases particularly Huntington's disease abnormal HDAC1 and HDAC2 activity impacts pathways involving neuroplasticity and neuron survival. Shared interactions with proteins like p53 highlight their significance in disease mechanisms.