Abcam, ab306108, PE Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377]

Size: 100µL
Rabbit Recombinant Monoclonal Topoisomerase II alpha antibody - conjugated to PE.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5377,
Isotype:IgG,
Conjugation:PE,
Excitation/Emission:Ex: 480;565nm, Em: 578nm,
Carrier free:No,
Applications:Antibody Labelling, Target Binding AffinitySee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
conjugated primary antibody
is "made to order" and it is released using a quantitative quality control method that ensures binding affinity and labelling efficiency of the conjugate. Via leveraging the power of the
Lightning-Link® conjugation technology
, Abcam will deliver highly consistent recombinant conjugates in <2 weeks, giving you access to an ever growing portfolio of antibody-label combinations.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.
How are conjugated primary antibodies validated?
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Topoisomerase II alpha and Topoisomerase II beta often referred to as TOP2A and TOP2B respectively are enzymes weighing approximately 170 kDa each. They are known for their role in altering the topological states of DNA during transcription and replication by creating transient double-stranded breaks in the DNA helix. TOP2A and TOP2B exhibit differential expression; TOP2A is generally more active in proliferating cells especially in the S and G2/M phases of the cell cycle while TOP2B is expressed in non-proliferating cells. The expression occurs in a variety of central tissues with notable presence in the liver lungs and brain.
Biological function summary
These enzymes are important for processes like DNA replication chromosome segregation and recombination. They don't function as part of larger complexes but perform their tasks independently. While both enzymes facilitate DNA strand passage TOP2A is more relevant during cell division ensuring that chromosomes are properly disentangled and segregated. Meanwhile TOP2B functions in regulating transcription in differentiated cells by enabling structural changes necessary for gene expression modulation.
Pathways
Topoisomerase II alpha and beta are integral to DNA replication and repair pathways. In the replication pathway they work closely with proteins like DNA polymerase to ensure DNA strands do not entangle. In the DNA damage response pathway these enzymes help resolve DNA supercoils and knots that form during replication stress. By interacting with proteins such as the breast cancer type 1 susceptibility protein (BRCA1) they contribute significantly to maintaining genome stability preventing genetic aberrations during replication.
Topoisomerase II enzymes have strong links to cancer and neurological disorders. In cancers particularly those involving rapidly dividing cells like leukemia overexpression or mutations in TOP2A can lead to unchecked cell proliferation. Interactions with proteins like p53 a tumor suppressor highlight their importance in cancer biology. In neurological disorders aberrant activity of TOP2B has connections to neurodegenerative diseases such as Alzheimer's through its role in transcription regulation. These interactions can affect proteins associated with neuronal function highlighting the enzymes' diverse impact on human health.