Abcam, ab306853, Human TBX2 knockout U-87 MG cell line

Size: 1000000Cells / vial / 2 x 1000000Cells / vial
TBX2 KO cell line available to order. KO validated by. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:U-87 MG,
Species or organism:Human,
Tissue:Brain,
Form:LiquidSee storage information,
Disease:Glioblastoma

Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TBX2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tbx2 also known as T-box 2 is a transcription factor with a molecular weight of approximately 80 kDa. This protein is a member of the T-box family which is known for regulating gene expression through DNA binding. Tbx2 plays a role in controlling developmental processes by suppressing the expression of certain target genes. It is expressed in various tissues including heart limbs and craniofacial regions during embryonic development.
Biological function summary
This transcription factor is influential in cellular proliferation and developmental growth. It acts as a transcriptional repressor and participates in developmental processes such as osteogenesis and limb formation. Tbx2 is commonly found in complexes with other proteins that help modulate its effects on gene expression particularly during the early stages of embryogenesis.
Pathways
T-box 2 interacts importantly within the Wnt signaling and Sonic Hedgehog (Shh) pathways. These pathways are vital in developmental signaling where Tbx2 works with proteins such as beta-catenin in Wnt signaling influencing gene transcription linked to cell division and differentiation. Tbx2's role in these pathways affects the expression of key genes involved in tissue patterning and development.
Tbx2 has connections to cancer and developmental disorders. Its overexpression can lead to unchecked cell proliferation implicating it in melanoma and other cancers. Additionally its role in developmental processes links Tbx2 to disorders such as Holt-Oram syndrome through interactions with Tbx5 another member of the T-box family. Understanding Tbx2's precise functions and interactions is essential for developing therapeutic strategies targeting these diseases.