Abcam, ab307551, Anti-SLC10A1/NTCP1 antibody [EPR27105-63] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SLC10A1/NTCP1 antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR27105-63,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SLC10A1 also known as NTCP1 (Na+/taurocholate co-transporting polypeptide) is an important membrane transporter responsible for the uptake of bile acids. It has a molecular mass of approximately 38 kDa. This protein is predominantly expressed in the liver where it is located on the basolateral membrane of hepatocytes. By facilitating the transport of sodium ions and bile acids NTCP1 plays a significant role in maintaining bile acid homeostasis within the body.
Biological function summary
NTCP1 is essential for hepatic bile acid uptake which is an important step in bile acid recycling and metabolism. It functions as a solitary transporter without forming complexes with other proteins. The protein intake bile salts and conjugates them with taurine and glycine. This process ensures the efficient cycling and detoxification of bile acids regulating their levels in the liver and bloodstream.
Pathways
The NTCP1 is critical in the enterohepatic circulation of bile acids a process tightly controlled within the body. It significantly influences pathways like the bile acid synthesis pathway where it works alongside other transporters such as ASBT (apical sodium-dependent bile acid transporter) and OSTα/OSTβ (organic solute transporter alpha/beta). Additionally NTCP1 is involved in the Farnesoid X Receptor (FXR) regulatory pathway which modulates bile acid synthesis regulating the expression of genes that encode for enzymes involved in the biosynthesis of bile acids.
Improper functioning of NTCP1 relates to liver diseases such as cholestasis. This condition results from impaired bile flow causing accumulation of bile acids that can damage liver cells. Another relevant disorder is hepatitis B infection. NTCP1 acts as a receptor for the hepatitis B virus mediating viral entry into hepatocytes. This interaction emphasizes its potential role in the development and progression of hepatitis B where proteins like hepatitis B surface antigens (HBsAg) engage with NTCP1 to facilitate the infection process.