Abcam, ab307565, Anti-5-methyl cytosine adenosine di-nucleoside (5-mCpA) antibody [2C8H8A6]

Size: 20µg / 100µg / 1mg
Mouse Recombinant Monoclonal 5-mCpA antibody. Suitable for Dot and reacts with Modified Nucleic Acid samples.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2C8H8A6,
Isotype:IgG1,
Carrier free:No,
Applications:DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
5-Methyl cytosine adenosine dinucleoside (5-mCpA) functions mechanically as an epigenetic modification. It constitutes a specific sequence where the cytosine base undergoes methylation forming a methyl group at the 5th carbon position. This modification takes place largely in genomic DNA where cytosine nucleosides occur beside adenosine nucleosides. 5-mCpA commonly appears in various cell types with higher expression regions observed specifically in gene regulatory domains in eukaryotic cells. The mass of 5-mCpA can differ depending on the arranged nucleotide framework containing the methyl group.
Biological function summary
The mechanism involving this dinucleoside affects gene expression regulation. 5-mCpA functions as part of a complex involved in chromatin remodeling. Additionally this complex interacts with other modified nucleotides such as 5-methyl cytosine. Its role is to influence the transcriptional activation or repression of associated genes by altering structural proteins that bind to the chromatin. The presence of these methylated nucleosides including methyl azide-modified versions indicates an important control mechanism in developmental processes and differentiation within organisms.
Pathways
5-mCpA plays an essential part in epigenetic regulation and the DNA methylation pathway. The DNA methylation pathway runs in parallel with histone modification to alter chromatin structure and gene accessibility. Proteins such as DNA methyltransferases are closely related to the formation and maintenance of 5-mCpA within these pathways. The interaction between methylation paths and transcription factors contributes significantly to controlling cell cycle progression and other cellular processes.
Alterations in 5-mCpA levels directly associate with cancer and neurological disorders. Aberrant DNA methylation patterns involving 5-mCpA can contribute to tumorigenesis as seen in certain cancer types where loss of methylation is observed. Similarly neurological conditions like Rett syndrome may relate to disruptions in this methylation process. Proteins like MECP2 which bind specifically to methylated DNA including 5-mCpA are implicated in these disorder pathways indicating a connection between altered epigenetic marks and disease manifestation.