Abcam, ab308061, Anti-SCAP antibody [EPR26242-60] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SCAP antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR26242-60,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SCAP also known as SREBP cleavage-activating protein is an important player in lipid homeostasis. SCAP has a molecular mass of approximately 150 kDa. It locates in the endoplasmic reticulum and Golgi apparatus within cells. The protein facilitates the movement of sterol regulatory element-binding proteins (SREBPs) from the endoplasmic reticulum to the Golgi. This action activates SREBPs by enabling their cleavage which is necessary for cholesterol and lipid synthesis regulation.
Biological function summary
SCAP acts as a cholesterol sensor when paired with SREBPs in a complex. The SCAP-SREBP complex remains inactive within the endoplasmic reticulum at high cholesterol concentrations. When cholesterol levels decrease SCAP aids in the transport of SREBPs to the Golgi apparatus. This transport triggers proteolytic activation of SREBPs which then migrate to the nucleus to promote gene expression for lipid metabolism.
Pathways
SCAP is instrumental in the cholesterol biosynthesis and lipid metabolic pathways. The protein interacts closely with SREBPs in these pathways to regulate lipid balance within cells. In particular SCAP's action is linked with the insulin signaling pathway aiding in the coordination of growth factor-mediated lipid synthesis. This highlights its importance in the control of lipid-related gene transcription together with proteins like Insig which anchor the SCAP-SREBP complex under high cholesterol levels.
SCAP plays a role in metabolic syndrome and cardiovascular diseases. Dysregulation of SCAP can lead to abnormal cholesterol levels contributing to atherosclerosis and other lipid disorders. The function of SCAP connects to proteins like HMG-CoA reductase in these conditions as it co-regulates the synthesis of cholesterol. Understanding SCAP's role in these diseases may open new therapeutic strategies for managing cholesterol-related disorders.