Abcam, ab308163, Anti-NPEPPS antibody [HL1531]

Size: 100µL
Rabbit Recombinant Monoclonal NPEPPS antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Dog, Human, Cat, Rat, Mouse, Zebrafish samples. Immunogen corresponding to Recombinant Fragment Protein within Human NPEPPS.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:HL1531,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human, Dog, Cat, Zebrafish,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NPEPPS. The exact immunogen used to generate this antibody is proprietary information.P55786

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NPEPPS also known as aminopeptidase puromycin sensitive or APP is a metalloprotease with an approximate molecular mass of 103 kDa. It functions by catalyzing the cleavage of N-terminal amino acids from peptides which is important for many cellular processes. NPEPPS localizes to both the cytoplasm and nucleus within cells and it shows widespread expression across various tissues especially in the brain heart and liver. Its activity plays an important role in the breakdown and recycling of intracellular proteins.
Biological function summary
NPEPPS influences protein turnover by participating in peptide degradation and processing. The enzyme associates with complexes involved in antigen processing impacting the production of antigenic peptides presented by MHC class I molecules. By regulating peptide availability NPEPPS facilitates immune responses and maintains cellular protein homeostasis. This regulatory effect makes it important for several physiological and cellular functions emphasizing its involvement beyond simple peptide cleavage.
Pathways
The NPEPPS enzyme influences the ubiquitin-proteasome system an essential cellular mechanism for protein degradation. This pathway requires NPEPPS for the final trimming of peptides before they are presented by the immune system. The enzyme's activity also connects to the mTOR signaling pathway which controls cell growth and metabolism. Proteins such as ubiquitin and components of the proteasome interact with NPEPPS linking its mechanical function to larger cellular and metabolic pathways.
NPEPPS has associations with neurodegenerative diseases like Alzheimer's disease and Huntington's disease. Altered enzyme function can affect peptide accumulation potentially contributing to the pathological protein aggregates observed in these diseases. Investigations also show a connection to proteins such as amyloid precursor protein implicating NPEPPS in pathways leading to amyloid plaque formation in Alzheimer's. These interactions highlight the potential impact of NPEPPS in disease mechanisms and as a target for therapeutic intervention.